Enzyme analysis and consultation
Enzymes are bio-catalysts that play a crucial role in the manufacture, preservation and improvement of everyday products, as well as in bio-based processes:
These include:
Food (drinks, bread, cheese, meat products)
Health (medication, dietary supplements)
Agriculture (feed processing, silage, veterinary medicine)
Cleaning supplies (detergents, cosmetics, toothpaste)
Waste management (biogas, biofuel, phosphate recycling)
Development
Measurement
Optimization
We offer scientific advice on the development of new enzyme products, on optimizing existing enzyme products and on ongoing testing/quality control of your enzyme-containing products.
With us you get:
- Research on the latest scientific findings regarding your question
- Analyzes that are specially adapted to your question
- Documentation and assessment of the collected data
- Recommendations for action based on the newly gained knowledge
Please email inquiries to: enzymes@amplab.de
Delivery address for enzyme samples:
AMP-Lab GmbH
Drechslerweg 21
55128 Mainz
Germany
Some enzymes and their uses
| Protein metabolism | ||
|---|---|---|
| Enzyme | EC No. | application in production of |
| Bromelain | 3.4.22.32/33 | foods, feed, beverages, pharmaceuticals |
| Papain | 3.4.22.2 | foods, cosmetics, pharmaceuticals |
| Trypsin | 3.4.21.4 | medicine, pharmaceuticals |
| Chymotrypsin | 3.4.21.1 | medicine, pharmaceuticals |
| Actinidain | 3.4.22.14 | foods, pharmaceuticals |
| Carbohydrate metabolism | ||
| Enzyme | EC No. | application in production of |
| Lactase | 3.2.1.23 | foods, feed, dairy products |
| Lysozyme | 3.2.1.17 | feed, foods, beverages, pharmaceuticals |
| α-Galactosidase | 3.2.1.22 | foods, feed |
| Cellulase, CMCase | 3.2.1.4 | textiles, detergents |
| Invertase | 3.2.1.26 | foods, feed |
| Glucoamylase | 3.2.1.3 | beverages, foods, sugar, biofuel |
| Xylanase | 3.2.1.8 | paper, foods, biofuel |
| Collagenase | 3.4.24.3 | medicine, pharmaceuticals, cosmetics |
| beta-Glucanase | 3.2.1.39 | biofuel |
| Lipid metabolism | ||
| Enzyme | EC No. | application in production of |
| Lipase | 3.1.1.3 | bakery products, feed, detergents |
| Oxido-Reductases | ||
| Enzyme | EC No. | application in production of |
| Catalase | 1.11.1.6 | foods, dairy products, paper, textiles |
| Glucose Oxidase | 1.1.3.4 | biofuel, foods |
| Phenole Oxidase | 1.10.3.1 | pharmaceuticals, foods |
| Diamino Oxidase | 1.4.3.6 | pharmaceuticals, foods |
| Phosphohydrolases | ||
| Enzyme | EC No. | application in production of |
| acidic Phosphatase | 3.1.3.2 | feed |
| alkaline Phosphatase | 3.1.3.1 | medicine |
| Phytase | 3.1.3.8/26 | feed, silage |
Activity assays of further enzymes on request: enzymes@amplab.de
Extraction, purification and characterization of enzymes
Our expertise in protein purification and characterization is based on scientific experience with enzymes of proteolysis and cell energy metabolism. Enzymes are essential for catalysis and the control of metabolism. As an approach for understanding enzyme function in vivo, we offer enzyme characterization under near physiological conditions. This is important for general pathophysiology, proteomic research and analysis of recombinant enzymes.
We offer:
- enzyme solubilization from natural or recombinant material by specific disintegration techniques
- gentle enzyme purification to homogeneity, preserving the native structure and function
- kinetic analysis of enzyme catalysis and its control under physiological conditions
- analysis of enzyme subunit aggregation and cell structure interaction, considering the significance in vivo
Catalytic Activity
Animal metabolism can be screened in homogenates or extracts of frozen tissues (e.g.brain, heart, liver, kidney, skeletal muscle, and gonads) by photometric or fluorimetric analyses of key enzymes and central metabolites.
The physiological capacities of the different metabolic pathways are determined by measuring the maximal catalytic activities of key enzymes (maximal velocity, Vmax under standard conditions).
You will receive a detailed protocol of measurement and our physiological interpretation of the data.
For further information please contact: enzymes@amplab.de
